التفاصيل البيبلوغرافية
| العنوان: |
Biofunctionalized Silicon Carbide-Titanium (SiC-Ti) Platforms for the Determination of Enteropathogenic Escherichia coli by an Infrared Biosensor. |
| المؤلفون: |
Gómez-Montaño, Francisco Javier, Reyes-Betanzo, Claudia, Orduña-Díaz, Abdú, Avelino-Flores, Fabiola, Retana-González, Ricardo Alan, Vázquez-Gutiérrez, Isabel Gudelia |
| المصدر: |
Analytical Letters; 2024, Vol. 57 Issue 5, p772-782, 11p |
| مستخلص: |
Escherichia coli (E. coli) may be harmful to humans. Hence, a rapid, low cost, and easy detection method is needed, and optical biosensors are an excellent option. Here a new platform based upon silicon carbide and titanium (SiC-Ti) was used to determine enteropathogenic E. coli by Fourier transform infrared (FTIR) spectroscopy. Self-assembly monolayer (SAM) approach was used to modify the platform. Antibody anti-E. coli flagella were obtained and utilized as the biological recognition element. Three concentrations of bacteria (10, 100, and 1000 CFU/mL) were determined. The detection region was from 1330 to 1480 cm−1, where characteristic bands associated with E. coli were determined. An increase in the absorption intensity was observed with the bacteria concentration, demonstrating suitability for quantitative analysis. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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