التفاصيل البيبلوغرافية
| العنوان: |
An Identification of Functional Genetic Variants in B4GALNT2 Gene and Their Association with Growth Traits in Goats. |
| المؤلفون: |
Xu, Liang, Chen, Zitong, Chen, Shuheng, Chen, Yu, Guo, Jiazhong, Zhong, Tao, Wang, Linjie, Zhan, Siyuan, Li, Li, Zhang, Hongping, Cao, Jiaxue |
| المصدر: |
Genes; Mar2024, Vol. 15 Issue 3, p330, 14p |
| مصطلحات موضوعية: |
GENETIC variation, GOATS, GOAT breeds, SINGLE nucleotide polymorphisms, HAPLOTYPES, GENETIC polymorphisms, GOAT diseases |
| مستخلص: |
β-1,4-N-acetylgalactosamine transferase 2 (B4GALNT2) is a vital candidate gene that affects the growth traits in sheep. However, whether it has the same function in goats remains to be investigated further. This study selected 348 Nanjiang Yellow goats, screened all exons, and conserved non-coding regions of the B4GALNT2 gene for single-nucleotide polymorphisms (SNPs). Our results revealed the presence of a synonymous mutation, rs672215506, within the exon of the B4GALNT2 gene in the Nanjiang Yellow goat population. The mutation resulted in a decrease in the mRNA stability of the B4GALNT2 gene. The results of SNP detection of the conserved non-coding region of the B4GALNT2 gene showed five potential regulatory SNPs in the Nanjiang Yellow goat population. Except for rs66095343, the ~500 bp fragments of the other four SNPs (rs649127714, rs649573228, rs652899012, and rs639183528) significantly increased the luciferase activity both in goat skeletal muscle satellite cells (MuSCs) and 293T cells. The genetic diversity indexes indicated low or intermediate levels for all six SNPs analyzed, and the genotype frequencies were in Hardy–Weinberg equilibrium. Association analysis showed that rs660965343, rs649127714, and rs649573228 significantly correlate with growth traits in the later stage of growth and development of Nanjiang Yellow goats. The haplotype combinations of H2H3 and H2H2 had higher body weight and greater body size. Moreover, H2H2 haplotype combinations significantly correlated with the litter size of the Nanjiang Yellow goats. The results of our study demonstrate the potential role of the B4GALNT2 gene as a functional genetic marker in the breeding programs of Nanjiang Yellow goats. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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