التفاصيل البيبلوغرافية
| العنوان: |
Differences in diacylglycerol acyltransferases expression patterns and regulation cause distinct hepatic triglyceride deposition in fish. |
| المؤلفون: |
Xiang, Xiaojun, Ji, Renlei, Han, Shangzhe, Xu, Xiang, Zhu, Si, Li, Yongnan, Du, Jianlong, Mai, Kangsen, Ai, Qinghui |
| المصدر: |
Communications Biology; 4/19/2024, Vol. 7 Issue 1, p1-12, 12p |
| مصطلحات موضوعية: |
LARIMICHTHYS, ACYLTRANSFERASES, RAINBOW trout, SCIAENIDAE, OLEIC acid |
| مستخلص: |
Triglyceride (TAG) deposition in the liver is associated with metabolic disorders. In lower vertebrate, the propensity to accumulate hepatic TAG varies widely among fish species. Diacylglycerol acyltransferases (DGAT1 and DGAT2) are major enzymes for TAG synthesis. Here we show that large yellow croaker (Larimichthys crocea) has significantly higher hepatic TAG level than that in rainbow trout (Oncorhynchus mykiss) fed with same diet. Hepatic expression of DGATs genes in croaker is markedly higher compared with trout under physiological condition. Meanwhile, DGAT1 and DGAT2 in both croaker and trout are required for TAG synthesis and lipid droplet formation in vitro. Furthermore, oleic acid treatment increases DGAT1 expression in croaker hepatocytes rather than in trout and has no significant difference in DGAT2 expression in two fish species. Finally, effects of various transcription factors on croaker and trout DGAT1 promoter are studied. We find that DGAT1 is a target gene of the transcription factor CREBH in croaker rather than in trout. Overall, hepatic expression and transcriptional regulation of DGATs display significant species differences between croaker and trout with distinct hepatic triglyceride deposition, which bring new perspectives on the use of fish models for studying hepatic TAG deposition. Two diacylglycerol acyltransferases were shown to be differently regulated in large yellow croaker and rainbow trout. While DGAT1 is increased upon oleic acid treatment more in croaker than in trout, DGAT2 showed no significant expression difference. [ABSTRACT FROM AUTHOR] |
|
Copyright of Communications Biology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Find this article in full text from Springer Verlag. 
Full Text Finder