دورية أكاديمية
Expanding boundaries - a cell biologist's guide to expansion microscopy.
| العنوان: | Expanding boundaries - a cell biologist's guide to expansion microscopy. |
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| المؤلفون: | Hümpfer, Nadja, Thielhorn, Ria, Ewers, Helge |
| المصدر: | Journal of Cell Science; Apr2024, Vol. 137 Issue 7, p1-12, 12p |
| مصطلحات موضوعية: | EXPANSION microscopy, OPTICAL diffraction, BIOLOGISTS, LIFE sciences, MICROSCOPY |
| مستخلص: | Expansion microscopy (ExM) is a revolutionary novel approach to increase resolution in light microscopy. In contrast to super-resolution microscopymethods that rely on sophisticated technological advances, including novel instrumentation, ExM instead is entirely based on sample preparation. In ExM, labeled target molecules in fixed cells are anchored in a hydrogel, which is then physically enlarged by osmotic swelling. The isotropic swelling of the hydrogel pulls the labels apart from one another, and their relative organization can thus be resolved using conventionalmicroscopes even if it was below the diffraction limit of light beforehand. As ExM can additionally benefit from the technical resolution enhancements achieved by super-resolution microscopy, it can reach into the nanometer range of resolution with an astoundingly lowdegree of error induced by distortion during the physical expansion process. Because the underlying chemistry is well understood and the technique is based on a relatively simple procedure, ExM is easily reproducible in non-expert laboratories and has quickly been adopted to address an ever-expanding spectrum of problems across the life sciences. In this Review, we provide an overview of this rapidly expanding new field, summarize the most important insights gained so far and attempt to offer an outlook on future developments. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: | Complementary Index |
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