دورية أكاديمية
Enhanced immune capture of extracellular vesicles with gelatin nanoparticles and acoustic mixing.
| العنوان: | Enhanced immune capture of extracellular vesicles with gelatin nanoparticles and acoustic mixing. |
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| المؤلفون: | Yiqian Mao, Juan Li, Jingxing Li, Cuicui Su, Kaixiang Long, Daojiang Li, Zhao Ding, Shishang Guo |
| المصدر: | Analyst; 6/7/2024, Vol. 149 Issue 11, p3195-3203, 9p |
| مصطلحات موضوعية: | EXTRACELLULAR vesicles, NANOPARTICLES, CANCER cells, SURFACE roughness, BIOMOLECULES, GELATIN |
| مستخلص: | Extracellular vesicles (EVs) originating from cancer cells incorporate various critical biomolecules that can aid in early cancer diagnosis. However, the rapid analysis of these micro vesicles remains challenging due to their nano-scale size and overlapping dimensions, hindering sufficient capture in terms of quantity and purity. In this study, an acoustofluidic device was developed to enhance the yield of immune-captured EVs. The channel of the device was modified with degradable gelatin nanoparticles (~220 nm) to increase the surface roughness, and subsequently treated with CD63 antibodies. The acoustic-induced streaming would prolong the rotation time of the EVs in the targeted continuous flow area, improving their aggregation towards the surrounding pillars and subsequent capture by the specific CD63 antibodies. Consequently, the capture efficiency of the device was improved when the signal was on, as evidenced by enhanced fluorescence intensity in the main channel. It is demonstrated that the acoustofluidic device could enhance the immune capture of EVs through acoustic mixing, showcasing great potential in the rapid and fast detection of EVs in liquid biopsy applications. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: | Complementary Index |
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