التفاصيل البيبلوغرافية
| العنوان: |
Mno-miR164a and MnNAC100 regulate the resistance of mulberry to Botrytis cinerea. |
| المؤلفون: |
Donghao Wang, Lin Chen, Chaorui Liu, Hairui Wang, Zixuan Liu, Xianling Ji, Ningjia He, Youchao Xin |
| المصدر: |
Physiologia Plantarum; Mar/Apr2024, Vol. 176 Issue 2, p1-12, 12p |
| مصطلحات موضوعية: |
BOTRYTIS cinerea, GENE expression, TRANSCRIPTION factors, MULBERRY, CULTIVARS, PLANT genes, PATHOGENIC fungi |
| مستخلص: |
Although microRNAs (miRNAs) regulate the defense response of a variety of plant species against a variety of pathogenic fungi, the involvement of miRNAs in mulberry's defense against Botrytis cinerea has not yet been documented. In this study, we identified responsive B. cinerea miRNA mno-miR164a in mulberry trees. After infection with B. cinerea, the expression of mno-miR164a was reduced, which was fully correlated with the upregulation of its target gene, MnNAC100, responsible for encoding a transcription factor. By using transient infiltration/VIGS mulberry that overexpressed mno-miR164a or knocked-down MnNAC100, our study revealed a substantial enhancement in mulberry's resistance to B. cinerea when mno-miR164a was overexpressed or MnNAC100 expression was suppressed. This enhancement was accompanied by increased catalase (CAT) activity and reduced malondialdehyde (MDA) content. In addition, mno-miR164a-mediated inhibition of MnNAC100 enhanced the expression of a cluster of defense-related genes in transgenic plants upon exposure to B. cinerea. Meanwhile, MnNAC100 acts as a transcriptional repressor, directly suppressing the expression of MnPDF1.2. Our study indicated that the mno-miR164a-MnNAC100 regulatory module manipulates the defense response of mulberry to B. cinerea infection. This discovery has great potential in breeding of resistant varieties and disease control. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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