التفاصيل البيبلوغرافية
| العنوان: |
Relationship between the bacterial ocular surface microbiota and outcomes for cats with feline herpesvirus type 1 ocular surface disease. |
| المؤلفون: |
Mills, Erinn P., Liu, Chin‐Chi, Mironovich, Melanie A., Taylor, Christopher M., Luo, Meng, Emelogu, Ugochi, Scott, Erin M., Leis, Marina L., Carter, Renee T., Camacho‐Luna, Pilar, Lewin, Andrew C. |
| المصدر: |
Veterinary Ophthalmology; Jul2024, Vol. 27 Issue 4, p318-329, 12p |
| مصطلحات موضوعية: |
BACTERIAL DNA, CATS, CAT diseases, POLYMERASE chain reaction, BACTERIAL diversity, FLEA control |
| مستخلص: |
Objective: Feline herpesvirus 1 (FHV‐1) causes ocular surface disease in domestic cats. The purpose of this study was to assess the relationship between bacterial ocular surface microbiota and outcomes for cats with FHV‐1 ocular surface disease. Animals Studied: Twenty‐two shelter‐housed cats with confirmed FHV‐1 ocular surface disease. Procedures: Animals were grouped according to FHV‐1 shedding and ocular clinical scores following intervention: worsened outcome (WorOut, n = 11) or improved outcome (ImpOut, n = 11). Scoring and conjunctival sampling were completed on Days 1 and 8 of twice daily antiviral treatment. Bacterial DNA was extracted and submitted for 16S rRNA gene sequencing. Real‐time polymerase chain reaction was performed for selected bacterial species. Overall DNA concentration between groups was assessed. Results: Bacterial microbiota relative abundance composition was significantly different between ImpOut and WorOut groups (weighted UniFrac p =.006). Alpha diversity was significantly higher in the ImpOut group compared with the WorOut group (Shannon p =.042, Simpson's p =.022, Pielou's p =.037). Differences in the relative abundance of various phyla and species were detected between groups. Total DNA concentration was higher in the WorOut group compared with the ImpOut group (p =.04). Feline GAPDH (p =.001) and Bilophila wadsworthia (p =.024) copy number was significantly higher in the ImpOut group compared with the WorOut group. Conclusions: The results highlight the important relationship between the bacterial ocular surface microbiota and FHV‐1 infection outcomes in cats treated with antiviral medications. Low bacterial species diversity, higher overall DNA (presumed predominantly bacterial) load, and certain bacterial phyla/species were associated with poor outcomes for cats with FHV‐1 ocular disease. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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