التفاصيل البيبلوغرافية
| العنوان: |
Plasmonic nanoparticle-assisted single-molecule dynamic binding for protein kinase activity digital counting. |
| المؤلفون: |
Qingnan Li, Wenzhi Qiang, Yanan Deng, Lehui Xiao |
| المصدر: |
Chemical Communications; 8/28/2024, Vol. 60 Issue 67, p8848-8851, 4p |
| مصطلحات موضوعية: |
PROTEIN kinases, CARRIER proteins, PROTEIN binding, PLASMONICS, MEDICAL screening |
| مستخلص: |
In this work, we design a new approach for digital counting-based protein kinase activity assay by using plasmonic nanoparticle-assisted single-molecule dynamic binding. This method breaks the concentration-dependent limitation in single-molecule detection and displays good selectivity and sensitivity with a detection limit as low as 0.0001 U mL-1 for the protein kinase A (PKA) assay, which would find broad applications in drug screening and medical investigations. [ABSTRACT FROM AUTHOR] |
|
Copyright of Chemical Communications is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
