التفاصيل البيبلوغرافية
| العنوان: |
苏铁叶枯病病原菌的分离鉴定及有效药物筛选. (Chinese) |
| Alternate Title: |
Isolation, identification and fungicide screening of Cycas leaf blight pathogen. (English) |
| المؤلفون: |
许恬, 黄大明, 李蓉, 黃怡婷, 李杏蔚, 张磊, 覃茜 |
| المصدر: |
Southwest China Journal of Agricultural Sciences; 2024, Vol. 37 Issue 7, p1513-1521, 9p |
| مصطلحات موضوعية: |
PROPICONAZOLE, MANCOZEB, ALTERNARIA, MOLECULAR biology, BOTANICAL gardens |
| Abstract (English): |
[Objective] The purpose of the study was to identify the cause of leaf blight in Cycas in the Qingxiu Mountain Cycas Garden of Nanning Botanical Garden, explore methods for prevention and control, and provide a theoretical basis for the effective protection of Cycas germplasm resources. [Method] 113 samples of blight leaves were collected from different diseased Cycas plants. The pathogenic fungi were isolated and purified using the dilution plating and single spore isolation method. Pathogenicity of the isolated strains was determined using Koch's postulates. Species identification of the isolates was carried out based on morphological and molecular biology methods. Seven fungicides, Mancozeb, Picoxystrobin, Propiconazole, Azoxystrobin, Fluopicolide Trifloxystrobin, Chlorfenapyr Pyriproxyfen, Chlorothalonil, were selected for in vitro efficacy tests against the pathogenic fungus. [Result] In the study, a total of 72 isolates were obtained through the isolation and purification of microorganisms from blight tissues, belonging to 13 different genera; Colletotrichum, Fusarium, Alternaria, Pestalotiopsis, Phyllosticta, Nigrospora, Cladosporium, Chaetomium, Hypoxylon, Xylaria, Botryosphaeria, Aspergilus, Neofusicoccum. Pathogenicity testing revealed that the Fusarium isolate GX2210 could induce lesions when inoculated into Cycas fronds. Further sequence analysis of the CaM, TUB, RPB2 and TEFla genes was performed and confirmed the identity of this strain as Fusarium incarnatum. Efficacy tests showed that Mancozeb was the most effective fungicide, with an inhibition concentration (ID50) of 0.3577 mg/L against the pathogen. [Conclusion] F. incarnatum is the pathogen causing the leaf blight of Cycas in Nanning Botanical Garden. Mancozeb has a good inhibitory effect on F. incarnatum, and can be considered for the prevention and control of cycad leaf blight. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
【目的】明确造成南宁植物园青秀山苏铁园内苏铁叶片干枯的病因,研究防治手段,为有效保护苏铁种质资源提供理论基 础。【方法】采集不同发病苏铁植株上的枯萎叶片样本113个,使用平板培养及稀释分离法将病原真菌分离纯化,通过柯赫氏法则 測定分离菌株致病力,依据形态学及分子生物学鉴定等手段对所获得分离株进行物种鉴定,选取代森锰锌、啶氧菌酯、苯甲・丙环 唑、苯甲・嘧菌酯、氟菌・肟菌酯、氯氟醚・吡唑酯和百菌清等7种杀菌剂对病原菌进行室内药效试验。【结果】通过对枯萎组织的微 生物分离纯化,共获得72株分离菌株,经ITS序列分析发现分别来自炭疽菌属、镰刀菌属、链格孢菌属、拟盘多毛孢属、叶点霉属、 黑孢霉属、枝孢菌属、球毛壳菌属、炭团菌属、炭角菌属、葡萄座腔菌属、曲霉属及小新壳梭孢属等13个不同属。对其中的真菌进 行致病力测定发现,镰刀菌属的GX2210菌株经针刺接种到苏铁羽片会产生枯萎病斑;进一步采用CaM、6TUB、RPB2和TEFla基 因序列分析,确定该菌株为变红镰孢菌(Fusarium incarnatum);药效试验显示,代森锰锌是抑制该菌株的最有效药物,抑制中量 (ID50)为0.3577 mg/L。【结论】造成南宁植物园苏铁叶枯病的病原菌为变红镰孢菌。代森锰锌对变红镰孢菌具有较好的抑制作 用,可考虑用于苏铁叶枯病防治。 [ABSTRACT FROM AUTHOR] |
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