التفاصيل البيبلوغرافية
| العنوان: |
TLC Identification of Yao Medicine Pileostegia tomentellal and Extraction Technology and Content Determination of Umbelliferone. |
| المؤلفون: |
Jiangcun WEI, Xiumei MA, Meiyan QIU, Bing QING, Jingrong LU, Hong LEI, Xiaodong HUANG, Wen ZHONG |
| المصدر: |
Medicinal Plant; Apr2024, Vol. 15 Issue 2, p15-17, 3p |
| مصطلحات موضوعية: |
GRADIENT elution (Chromatography), ULTRAVIOLET lamps, SILICA gel, PHOSPHORIC acid, REFERENCE sources |
| مستخلص: |
[Objectives] To establish a TLC and content determination method of Pileostegia tomentellal, with umbelliferone as the indicator component. [Methods] TLC identification was performed by silica gel G thin layer plate with n-hexane-ethyl acetate (4:3) as the developing agent, and the plate was examined by UV lamp (365 nm). The umbelliferone content was determined by HPLC; Inertsil ODS-3 C18 column (4.60 mm x 250 mm, 5 µm); mobile phase acetonitrile-0.2% phosphoric acid gradient elution; detection wavelength 320 nm, flow rate 1.0 mL/min, column temperature 30 °C, injection volume 10 µL. [Results] The chromatogram of P. tomentellal showed the same color spot in the same position as that of reference medicinal material, and the spot was clear with good specificity. Umbelliferone showed a good linear relationship when the injection volume was 2.63 - 131.27 µg/mL (R² = 0.999 7). The average recovery of umbelliferone in the low, middle and high adding groups of P. tomentellal was 99.57% and the RSD was 2.15%. [Conclusions] The method can effectively identify Yao medicine P. tomentellal and accurately determine the content of umbelliferone in medicinal materials, which will provide a scientific basis for the development and utilization of medicinal resources of Yao medicine P. tomentellal. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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