التفاصيل البيبلوغرافية
| العنوان: |
Ligation of Leukocyte Function-Associated (LFA) Molecule-1 Provides an Accessory Signal for T-Cell Activation with Pokeweed Mitogen. |
| المؤلفون: |
Wallays, G., Ceuppens, J. L. |
| المصدر: |
Scandinavian Journal of Immunology; Feb1994, Vol. 39 Issue 2, p137-143, 7p, 3 Charts, 4 Graphs |
| مصطلحات موضوعية: |
LEUCOCYTES, PHAGOCYTES, RETICULO-endothelial system, LYMPHOCYTES, T cells, CELL adhesion molecules, ANTIGEN presenting cells, BLOOD cells, CELL growth, CYTOKINES, TUMOR necrosis factors |
| مستخلص: |
The ligation between Leucocyte-Function-Associated Molecule 1 (LFA-1) and Intercellular Adhesion Molecules (ICAM) is thought to be an important component in the stimulatory interaction between antigen-presenting cells (APC) and T cells. Similar to antigenic stimulation, T-cell stimulation with pokeweed mitogen (PWM) is highly dependent on monocytes as accessory cells. This is partly a consequence of the requirement for mitogen presentation by the monocytes. The study described here addressed the question of whether LFA-1 ligation by accessory cells influences the activation of T cells with PWM. To avoid multiple costimulatory interactions between T cells and monocytes, experiments were performed with purified T cells, which were stimulated with PWM bound on autologous red blood cells (PWM-RBC). Binding on the RBC substituted partly for PWM presentation by the monocytes. Anti-LFA-1 MoAbs were presented in the immobilized form in order to mimick LFA-1 ligation by cell-bound ICAM. Three out of three different MoAbs against the β-chain of the LFA-1 molecule (CD18) and two out of three MoAbs against the α-chain (CD11a) had an enhancing effect on T-cell proliferation. Proliferation was increased further by simultaneous addition of interleukin (IL)-1β and IL-6. Ligation of the LFA-1 molecule was found to enhance IL-2 production and tumour necrosis factor-α (TNF-α) production. The results suggest that interaction of LFA-1 with ICAM on the monocytes contributes to the accessory signal activity of monocytes in T-cell activation with PWM. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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