دورية أكاديمية
Ligation of Leukocyte Function-Associated (LFA) Molecule-1 Provides an Accessory Signal for T-Cell Activation with Pokeweed Mitogen.
العنوان: | Ligation of Leukocyte Function-Associated (LFA) Molecule-1 Provides an Accessory Signal for T-Cell Activation with Pokeweed Mitogen. |
---|---|
المؤلفون: | Wallays, G., Ceuppens, J. L. |
المصدر: | Scandinavian Journal of Immunology; Feb1994, Vol. 39 Issue 2, p137-143, 7p, 3 Charts, 4 Graphs |
مصطلحات موضوعية: | LEUCOCYTES, PHAGOCYTES, RETICULO-endothelial system, LYMPHOCYTES, T cells, CELL adhesion molecules, ANTIGEN presenting cells, BLOOD cells, CELL growth, CYTOKINES, TUMOR necrosis factors |
مستخلص: | The ligation between Leucocyte-Function-Associated Molecule 1 (LFA-1) and Intercellular Adhesion Molecules (ICAM) is thought to be an important component in the stimulatory interaction between antigen-presenting cells (APC) and T cells. Similar to antigenic stimulation, T-cell stimulation with pokeweed mitogen (PWM) is highly dependent on monocytes as accessory cells. This is partly a consequence of the requirement for mitogen presentation by the monocytes. The study described here addressed the question of whether LFA-1 ligation by accessory cells influences the activation of T cells with PWM. To avoid multiple costimulatory interactions between T cells and monocytes, experiments were performed with purified T cells, which were stimulated with PWM bound on autologous red blood cells (PWM-RBC). Binding on the RBC substituted partly for PWM presentation by the monocytes. Anti-LFA-1 MoAbs were presented in the immobilized form in order to mimick LFA-1 ligation by cell-bound ICAM. Three out of three different MoAbs against the β-chain of the LFA-1 molecule (CD18) and two out of three MoAbs against the α-chain (CD11a) had an enhancing effect on T-cell proliferation. Proliferation was increased further by simultaneous addition of interleukin (IL)-1β and IL-6. Ligation of the LFA-1 molecule was found to enhance IL-2 production and tumour necrosis factor-α (TNF-α) production. The results suggest that interaction of LFA-1 with ICAM on the monocytes contributes to the accessory signal activity of monocytes in T-cell activation with PWM. [ABSTRACT FROM AUTHOR] |
Copyright of Scandinavian Journal of Immunology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
قاعدة البيانات: | Complementary Index |
كن أول من يترك تعليقا!