التفاصيل البيبلوغرافية
| العنوان: |
Reversal of myocardial injury using genetically modulated human skeletal myoblasts in a rodent cryoinjured heart model |
| المؤلفون: |
Ye, Lei, Haider, Husnain Kh., Jiang, Shujia, Ling, Lieng H., Ge, Ruowen, Law, Peter K., Sim, Eugene K.W. |
| المصدر: |
European Journal of Heart Failure; Oct2005, Vol. 7 Issue 6, p945-952, 8p |
| مصطلحات موضوعية: |
MYOCARDIUM, MYOBLAST transfer therapy, CELLULAR therapy, THERAPEUTICS, HEART diseases, RODENTS, WOUNDS & injuries |
| مستخلص: |
Abstract: Background: We hypothesized that combination therapy using human myoblasts and VEGF165 will lead to better prognosis in a failing heart. Methods: Forty-eight female Wistar rats with cryoinjured hearts were randomized into non-treated normal (group-1, n =12), DMEM injected (group-2, n =10), myoblast-transplanted (group-3, n =12) and myoblast–hVEGF165 (group-4, n =14). Ten days after cryoinjury, 200 μl DMEM containing 3×106 cells or without cells was injected into the injured myocardium. Animals were maintained on cyclosporine for 6 weeks post cell transplantation. Heart function was assessed by echocardiography. Animals were sacrificed and hearts were processed for histochemical and immunohistochemical studies. Results: Histological examination showed survival of the donor myoblasts expressing lac-z and hVEGF165 in rat cardiac tissue. Fluorescent immunostaining for vWillebrand Factor-VIII and smooth muscle actin expression at low power microscope (×100) showed significantly higher blood vessel density in group-4 (31.25±1.82; 24.63±0.92) as compared to group-2 (13.29±1.0; p <0.001; 9.71±0.81, p <0.001) and group-3 (16.50±1.43, p <0.001; 14.5±1.34, p <0.001). Echocardiography showed that ejection fraction and fractional shortening of group-3 (93.36±1.52%, p =0.005; 75±3.75%, p =0.024) and group-4 (94.8±1.62%, p =0.003; 76.13±2.15%, p =0.011) significantly improved as compared to group-2 (81.8±3.3%, 55.1±7.18%). Conclusion: Myoblasts carrying of hVEGF165 are potential therapeutic transgene carriers for cardiac repair. [Copyright &y& Elsevier] |
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| قاعدة البيانات: |
Complementary Index |
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