التفاصيل البيبلوغرافية
| العنوان: |
Adoptive transfer of nontransgenic mesenteric lymph node cells induces colitis in athymic HLA-B27 transgenic nude rats. |
| المؤلفون: |
Hoentjen, F., Tonkonogy, S. L., Liu, B., Sartor, R. B., Taurog, J. D., Dieleman, L. A. |
| المصدر: |
Clinical & Experimental Immunology; Mar2006, Vol. 143 Issue 3, p474-483, 10p, 2 Charts, 6 Graphs |
| مصطلحات موضوعية: |
COLITIS, COLON diseases, LYMPH nodes, LYMPHATICS, LABORATORY rats, TRANSGENIC animals |
| مستخلص: |
HLA-B27 transgenic (TG) rats develop spontaneous colitis when colonized with intestinal bacteria, whereas athymic nude (rnu/rnu) HLA-B27 TG rats remain disease free. The present study was designed to determine whether or not HLA-B27 expression on T cells is required for development of colitis after transfer of mesenteric lymph node (MLN) cells into rnu/rnu HLA-B27 recipients. Athymic nontransgenic (non-TG) and HLA-B27 TG recipients received MLN cells from either TG or non-TG rnu/+ heterozygous donor rats that contain T cells. HLA-B27 TG rnu/rnu recipients receiving either non-TG or TG MLN cells developed severe colitis and had higher caecal MPO and IL-1β levels, and their MLN cells produced more IFN-γ and less IL-10 after in vitro stimulation with caecal bacterial lysate compared to rnu/rnu non-TG recipients that remained disease free after receiving either TG or non-TG cells. Interestingly, proliferating donor TG T cells were detectable one week after adoptive transfer into rnu/rnu TG recipients but not after transfer into non-TG recipients. T cells from either non-TG or TG donors induce colitis in rnu/rnu TG but not in non-TG rats, suggesting that activation of effector T cells by other cell types that express HLA-B27 is pivotal for the pathogenesis of colitis in this model. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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