التفاصيل البيبلوغرافية
| العنوان: |
THE HISTOLOGICAL LOCALIZATION OF FasL mRNA IN HUMAN LUMBAR DISC TISSUE. |
| المؤلفون: |
Ye, Fagang, Si, Chunxiang, Qiu, Yujin, Chen, Bohua, Lu, Zhenhua, Xia, Renyun |
| المصدر: |
Journal of Musculoskeletal Research; Jun2006, Vol. 10 Issue 2, p89-94, 6p, 3 Color Photographs, 1 Graph |
| مصطلحات موضوعية: |
MESSENGER RNA, LUMBAR vertebrae diseases, APOPTOSIS, LUMBAR vertebrae, SPINE, CELL death, CELLS |
| مستخلص: |
Lumbar disc has become a degenerative tissue since teenage. Apoptosis plays an important role in the processes of degeneration. FasL, a ligand of Fas receptor (a trans-membrane protein) promote signal transduction of apoptosis. In this study, fetal disc and degenerative disc from adults were examined for FasL mRNA expression. hPBMC was first treated by PHA-P, a fragment of FasL cDNA was then synthesized through one-step RT-PCR. Verified by sequencing and recombinant plasmids linearizing, the DIG-cRNA probe was synthesized by in vitro transcription. The distribution of FasL mRNA was observed on lumbar intervertebral disc specimens using in situ hybridization. FasL mRNA signals were expressed in both notochord cells and chondrocyte-like cells in the nucleus of fetal discs. In degenerative disc, the quantity of intervertebral disc cells reduced dramatically and the expression of FasL mRNA was not detected. Expression of FasL in fetal disc implied that Fas/FasL triggered cell death signal pathway involved in lumbar disk transition and degeneration. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
