التفاصيل البيبلوغرافية
| العنوان: |
Initial Phases of DNA Rehydration by NMR and Sorption Isotherm. |
| المؤلفون: |
Haranczyk, H., Czak, J., Nowak, P., Niziol, J. |
| المصدر: |
Acta Physica Polonica: A; Feb2010, Vol. 117 Issue 2, p397-402, 6p, 8 Graphs |
| مصطلحات موضوعية: |
SALMON, DNA, ATMOSPHERIC temperature, HUMIDITY, FREEZE-drying, ABSORPTION |
| مستخلص: |
The initial stages of rehydration of salmon sperm deoxyribonucleic acid (DNA) lyophilizates were observed using hydration kinetics, sorption isotherm, and high power proton relaxometry (at 30 MHz). The hydration kinetics reveals (i) a very tightly bound water not removed by incubation over silica gel (Ah 0 = 0:057±0:010), (ii) a tightly bound water [saturating at Ah 1 = 0:149±0:007, hydration time th 1 = (0:27±0:08) h], a tightly bound water (iii) [saturating at Ah 2 = 0:694 ± 0:039, with the hydration time th 2 = (9:8 ± 3:2) h], and (iv) a loosely bound water fraction for the samples hydrated at p/p0 ⩾ 76% [with the hydration time th 3 = (44 ± 14) h, and the contribution progressively increasing with the air humidity]. For the hydration at p/p0 = 100%, after t0 = (244 ± 22) h of incubation the swelling process begins. The amount of additional water uptake at swelling depended on the macrostructure of the sample. Sorption isotherm is sigmoidal in form and is fitted well by the Dent model with the mass of water saturating primary binding sites ΔM/m0 = 0.114. Proton free induction decay is a superposition of the immobilized proton signal (Gaussian, with T* 2S ≈ 20 μs) and two liquid signal components coming from tightly bound (T* 2L1 ≈ 100 μs, with the mass saturating at Δm/m0 = 0:111 ± 0:044) and loosely bound water fraction (with the amplitude proportional to the mass of water added). [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
