التفاصيل البيبلوغرافية
| العنوان: |
Schistosoma japonicum: screening of cercariae cDNA library by specific single-chain antibody against SIEA26-28 ku and immunization experiment of the recombinant plasmids containing the selected genes. |
| المؤلفون: |
Dong-mei Gao, Shi-ping Wang, Zhuo He, Ming-chiu Fung, Ming-she Liu, Lu-xin Yu, Xiu-chun Chen |
| المصدر: |
Parasitology Research; Jun2010, Vol. 107 Issue 1, p127-134, 8p, 3 Color Photographs, 1 Black and White Photograph, 4 Charts, 3 Graphs |
| مصطلحات موضوعية: |
SCHISTOSOMA japonicum, SCHISTOSOMIASIS, DNA vaccines, VACCINES, IMMUNOGLOBULINS, PLASMIDS |
| مستخلص: |
To obtain the gene encoding SIEA26-28 ku, which has been proven to be a potential anti-schistosomiasis vaccine candidate, screening Schistosoma japonicum ( Sj) cercariae cDNA library with soluble specific single-chain antibody (SIEA26-28 ku-scFv) was performed. A large amount of specific single-chain antibody was harvested through construction of recombinant expression vector pET32a/scFv. The protein was purified and characterized. By using this protein (PET32a-scFv) as a probe, S. japonicum cercariae cDNA library was screened. Two strong positive clones were selected, and their eukaryotic recombinant plasmids were constructed. These genes were named as S. japonicum ribosomal protein S4 ( SjRPS4) and S. japonicum ribosomal protein L7 ( SjRPL7), respectively. Experiments of mice showed that both SjRPS4 and SjRPL7 DNA vaccines could induce significant immunoprotection. Result of these experiments further proved that the specific single-chain antibody is a very valuable tool in screening of cDNA library to get the corresponding molecules. [ABSTRACT FROM AUTHOR] |
|
Copyright of Parasitology Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Find this article in full text from Springer Verlag. 
Full Text Finder