التفاصيل البيبلوغرافية
| العنوان: |
Synthesis of theanine from glutamic acid γ-methyl ester and ethylamine catalyzed by Escherichia coli having γ-glutamyltranspeptidase activity. |
| المؤلفون: |
Fei Zhang, Qing-Zhong Zheng, Qing-Cai Jiao, Jun-Zhong Liu, Gen-Hai Zhao |
| المصدر: |
Biotechnology Letters; Aug2010, Vol. 32 Issue 8, p1147-1150, 4p |
| مصطلحات موضوعية: |
GLUTAMIC acid, ESCHERICHIA coli, ETHYLAMINES, GLUTAMINE, THEANINE |
| مستخلص: |
Glutamic acid γ-methyl ester (GAME) was used as substrate for theanine synthesis catalyzed by Escherichia coli cells possessing γ-glutamyltranspeptidase activity. The yield was about 1.2-fold higher than with glutamine as substrate. The reaction was optimal at pH 10 and 45°C, and the optimal substrate ratio of GAME to ethylamine was 1:10 (mol/mol). With GAME at 100 mmol, 95 mmol theanine was obtained after 8 h. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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