دورية أكاديمية
Protein interaction network topology uncoversmelanogenesis regulatory network componentswithin functional genomics datasets.
| العنوان: | Protein interaction network topology uncoversmelanogenesis regulatory network componentswithin functional genomics datasets. |
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| المؤلفون: | Hsiang Ho, Milenkovic, Tijana, Memiševic, Vesna, Aruri, Jayavani, Przulj, Nataša, Ganesan, Anand K. |
| المصدر: | BMC Systems Biology; 2010, Vol. 4, p84-96, 13p |
| مصطلحات موضوعية: | PROTEIN-protein interactions, RNA, PHENOTYPES, GENOMICS |
| مستخلص: | Background: RNA-mediated interference (RNAi)-based functional genomics is a systems-level approach to identify novel genes that control biological phenotypes. Existing computational approaches can identify individual genes from RNAi datasets that regulate a given biological process. However, currently available methods cannot identify which RNAi screen "hits" are novel components of well-characterized biological pathways known to regulate the interrogated phenotype. In this study, we describe a method to identify genes from RNAi datasets that are novel components of known biological pathways. We experimentally validate our approach in the context of a recently completed RNAi screen to identify novel regulators of melanogenesis. Results: In this study, we utilize a PPI network topology-based approach to identify targets within our RNAi dataset that may be components of known melanogenesis regulatory pathways. Our computational approach identifies a set of screen targets that cluster topologically in a human PPI network with the known pigment regulator Endothelin receptor type B (EDNRB). Validation studies reveal that these genes impact pigment production and EDNRB signaling in pigmented melanoma cells (MNT-1) and normal melanocytes. Conclusions: We present an approach that identifies novel components of well-characterized biological pathways from functional genomics datasets that could not have been identified by existing statistical and computational approaches. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: | Complementary Index |
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