دورية أكاديمية
Expression of Wild-Type CFTR Suppresses NF-κB-Driven Inflammatory Signalling.
| العنوان: | Expression of Wild-Type CFTR Suppresses NF-κB-Driven Inflammatory Signalling. |
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| المؤلفون: | Hunter, Mairi J., Treharne, Kate J., Winter, Alexandra K., Cassidy, Diane M., Land, Stephen, Mehta, Anil |
| المصدر: | PLoS ONE; 2010, Vol. 5 Issue 7, p1-9, 9p, 3 Graphs |
| مصطلحات موضوعية: | CYSTIC fibrosis, INFLAMMATION, ANTI-inflammatory agents, TUMOR necrosis factors, GENETIC mutation, GENE transfection, ION channels, EPITHELIUM, CELL lines |
| مستخلص: | Background: Mutation of the cystic fibrosis transmembrane-conductance regulator (CFTR) causes cystic fibrosis (CF) but not all CF aspects can easily be explained by deficient ion transport. CF-inflammation provides one example but its pathogenesis remains controversial. Here, we tested the simple but fundamental hypothesis that wild-type CFTR is needed to suppress NF-κB activity. Methodology/Principal Findings: In lung epithelial (H441) and engineered (H57) cell lines; we report that inflammatory markers are significantly suppressed by wild-type CFTR. Transient-transfection of wild-type CFTR into CFTR-naïve H441 cells, dose-dependently down-regulates both basal and Tumour Necrosis Factor-α evoked NF-κB activity when compared to transfection with empty vector alone (p<0.01, n>5). This effect was also observed in CFTR-naïve H57-HeLa cells which stably express a reporter of NF-κB activity, confirming that the CFTR-mediated repression of inflammation was not due to variable reporter gene transfection efficiency. In contrast, H57 cells transfected with a control cyano-fluorescent protein show a significantly elevated basal level of NF-κB activity above control. Initial cell seeding density may be a critical factor in mediating the suppressive effects of CFTR on inflammation as only at a certain density (1×105 cells/well) did we observe the reduction in NF-κB activity. CFTR channel activity may be necessary for this suppression because the CFTR specific inhibitor CFTR |
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| قاعدة البيانات: | Complementary Index |
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