التفاصيل البيبلوغرافية
| العنوان: |
Distribution of genes encoding erythromycin ribosomal methylases and an erythromycin efflux pump in epidemiologically distinct groups of staphylococci. |
| المؤلفون: |
Eady, E. Anne, Ross, Jeremy I., Tipper, Joanne L., Walters, Christina E., Cove, Jonathan H., Noble, William C., Eady, E A, Ross, J I, Tipper, J L, Walters, C E, Cove, J H, Noble, W C |
| المصدر: |
Journal of Antimicrobial Chemotherapy (JAC); Feb1993, Vol. 31 Issue 2, p211-217, 7p |
| مصطلحات موضوعية: |
ANIMALS, CONTINUOUS ambulatory peritoneal dialysis, CYTOPLASM, DNA probes, DOGS, DRUG resistance in microorganisms, ERYTHROMYCIN, GENES, IMMUNITY, STAPHYLOCOCCAL diseases, STAPHYLOCOCCUS, SWINE, TRANSFERASES, PHENOTYPES, PHARMACODYNAMICS |
| مستخلص: |
Erythromycin-resistant staphylococci can be divided into two phenotypic classes based on their pattern of cross-resistance to other macrolides, lincosamides and type B streptogramins. Strains inducibly or constitutively resistant to all MLS antibiotics possess erythromycin ribosomal methylase (erm) genes, whereas strains inducibly resistant to only 14 and 15-membered ring macrolides and type B streptogramins harbour msrA, which encodes an ATP-dependent efflux pump. Dot-blot hybridization was used to study the distribution of ermA, ermB, ermC and msrA in five epidemiologically distinct groups of staphylococci. The most widely-distributed resistance determinant was ermC, which was detected in 112 (50.6%) of 221 isolates, alone in 106 isolates and in combination with a second erythromycin resistance determinant in six strains. MsrA was detected in 73 (33%) of isolates, alone in 65 and in combination with a methylase gene in eight strains. This determinant was responsible for erythromycin resistance in over one-third (36.4%) of clinical isolates of coagulase-negative staphylococci. ErmA and ermB were present in only a minority of isolates (5.9 and 7.2% of strains, respectively). The resistance determinants present in ten strains did not hybridize to any of the four probes although, in all cases, their resistance phenotype was consistent with the possession of a methylase gene. Interestingly, ermB was found exclusively in animal isolates of Staphylococcus intermedius, Staphylococcus xylosus and Staphylococcus hyicus, but not in coagulase-negative staphylococci of human origin. This determinant has previously only been found in a small number of epidemiologically related strains of Staphylococcus aureus. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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