التفاصيل البيبلوغرافية
| العنوان: |
Correlações entre atividade inseticida e resistência a proteólise de duas lectinas vegetais glicose/manose. (Portuguese) |
| Alternate Title: |
Relationship of Insecticidal Activity and Resistance to Proteolysis of Two Glucose/ Mannose Plant Lectins. (English) |
| المؤلفون: |
de Freitas, Cleverson Diniz Teixeira, Ramos, Marcio Viana, Souza, Diego Pereira, Marinho-Filho, Jose Delano Barreto, Teixeira, Fabiano Moura, de Oliveira, Jefferson Soares |
| المصدر: |
Comunicata Scientiae; 2011, Vol. 2 Issue 1, p34-41, 8p, 3 Black and White Photographs, 2 Charts, 3 Graphs |
| مصطلحات موضوعية: |
PROTEOLYSIS, GLUCOSE, MANNOSE, PLANT lectins, CANAVALIA, INSECT-plant relationships |
| Abstract (English): |
Purified seed lectins from the legumes Canavalia brasiliensis (ConBr) and Cratylia floribunda (CFL) were tested for insecticidal activity against Callosobruchus maculatus (Coleoptera: Bruchidae) and Dysdercus peruvianus (Hemiptera: Pyrrhocoridae). ConBr and CFL caused negative effects on larvae of C. maculatus at levels of 1% and 2% (w/w). Time of development of C. maculatus insects, grown in artificial seeds containing both lectins, was not significantly altered, but the emergence was slightly diminished. Both lectins were resisted to proteolysis by gut proteases of C. maculatus larvae. Artificial diets containing 1% and 2% of ConBr or CFL caused no effect on weight and survival of D. peruvianus nymphs. Gut proteases of D. peruvianus were able to breakdown ConBr and CFL after 2 hours of incubation. The ability of insects to digest the proteins was correlated directly with the deleterious effect observed. [ABSTRACT FROM AUTHOR] |
| Abstract (Portuguese): |
Lectinas purificadas de sementes de Canavalia brasiliensis (ConBr) e Cratylia floribunda (CFL) foram testadas quanto a atividade inseticida sobre Callosobruchus maculatus (Coleoptera: Bruchidae) e Dysdercus peruvianus (Hemiptera: Pyrrhocoridae). ConBr e CFL causaram efeitos deletérios sobre o desenvolvimento das larvas de C. maculatus nas concentrações de 1% e 2% (p/p). O tempo de desenvolvimento dos insetos alimentados com as sementes artificiais contendo ConBr ou CFL não foi significativamente alterado, entretanto a porcentagem de emergência diminuiu. Ambas lectinas foram resistentes a ação hidrolítica de proteases intestinais de larvas de C. maculatus. Dietas artificiais contendo as lectinas não causaram efeitos deletérios sobre a massa corporal e a sobrevivência de ninfas de D. peruvianus. Proteases intestinais extraídas de D. peruvianus foram capazes de hidrolisar ConBr e CFL após 2 horas de incubação. A habilidade dos insetos para digerir as lectinas parece estar diretamente relacionada ao efeito inseticida observado. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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