دورية أكاديمية
Differential Expression of Folate Receptor-Alpha, Sodium-Dependent Multivitamin Transporter, and Amino Acid Transporter (B (0, +)) in Human Retinoblastoma (Y-79) and Retinal Pigment Epithelial (ARPE-19) Cell Lines.
العنوان: | Differential Expression of Folate Receptor-Alpha, Sodium-Dependent Multivitamin Transporter, and Amino Acid Transporter (B (0, +)) in Human Retinoblastoma (Y-79) and Retinal Pigment Epithelial (ARPE-19) Cell Lines. |
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المؤلفون: | Jwala, Jwala, Vadlapatla, Ramya Krishna, Vadlapudi, Aswani Dutt, Boddu, Sai Hanuman Sagar, Pal, Dhananjay, Mitra, Ashim K. |
المصدر: | Journal of Ocular Pharmacology & Therapeutics; Jun2012, Vol. 28 Issue 3, p237-244, 8p |
مصطلحات موضوعية: | GENE expression, FOLIC acid, AMINO acid transport, EPITHELIAL cells, RETINOBLASTOMA, POLYMERASE chain reaction |
مستخلص: | Purpose: The overall objective of this study was to investigate the differential expression of folate receptor-alpha (FR-α), sodium-dependent multivitamin transporter (SMVT), and amino acid transporter [B (0, +)] in retinoblastoma (Y-79) and retinal pigment epithelial (ARPE-19) cells. Methods: Polymerase chain reaction (PCR) analysis was performed to confirm the existence of FR-α, SMVT, and B (0, +) in Y-79 and ARPE-19 cell lines. Quantitative real-time PCR was also performed to determine the relative expression of FR-α, SMVT, and B (0, +) at mRNA level in these cell lines. Quantitative uptake of [3H] Folic acid, [3H] Biotin, and [14C] Arginine was studied in Y-79 and ARPE-19 cells. Further, saturation kinetics of [3H] Folic acid, [3H] Biotin, and [14C] Arginine was performed in the presence of various concentrations of respective cold substrates to determine the kinetic parameters ( K |
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قاعدة البيانات: | Complementary Index |
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