التفاصيل البيبلوغرافية
| العنوان: |
ATL>pH-Dependent channel activity of heterologously-expressed main intrinsic protein (MIP) from rat lens. |
| المؤلفون: |
Drake, K. Dawn, Schuette, Diana, Chepelinsky, Ana B., Jacob, Tim J., Crabbe, M. James C. |
| المصدر: |
FEBS Letters; Feb2002, Vol. 512 Issue 1-3, p199, 6p |
| مصطلحات موضوعية: |
BACULOVIRUSES, LEUKEMIA, ERYTHROCYTE membranes |
| مستخلص: |
Wild-type rat lens main intrinsic protein (MIP) was heterologously expressed in the membrane of Spodoptera frugiperda (Sf21) cells using the baculovirus expression system and in mouse erythroid leukaemia cells (MEL C88). Both MEL and Sf21 cell lines expressing wild-type MIP were investigated for the conductance of ions using a whole cell patch clamp technique. An increase in conductance was seen in both expression systems, particularly on lowering the pH to 6.3. In Sf21 cells, addition of antibodies to the NPA1 box resulted in a reduction of current flow. These results suggest that MIP has pH-dependent ion channel activity, which involves the NPA1 box domain. [Copyright &y& Elsevier] |
|
Copyright of FEBS Letters is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder