التفاصيل البيبلوغرافية
| العنوان: |
ADF/cofilin mediates actin cytoskeletal alterations in LLC-PK cells during ATP depletion. |
| المؤلفون: |
Ashworth, Sharon L., Southgate, Erica L., Sandoval, Ruben M., Meberg, Peter J., Bamburg, James R., Molitoris, Bruce A. |
| المصدر: |
American Journal of Physiology: Renal Physiology; Apr2003, Vol. 53 Issue 4, pF852, 11p, 7 Color Photographs, 27 Black and White Photographs, 4 Diagrams, 2 Graphs |
| مصطلحات موضوعية: |
ACTIN, ISCHEMIA, CYTOSKELETON |
| مستخلص: |
Ischemic injury induces actin cytoskeleton disruption and aggregation, but mechanisms affecting these changes remain unclear. To determine the role of actin-depolymerizing factor (ADF)/ cofilin participation in ischemic-induced actin cytoskeletal breakdown, we utilized porcine kidney cultured cells, LLCPK[sub A4.8], and adenovirus containing wild-type (wt), constitutively active, and inactive Xenopus ADF/cofilin linked to green fluorescence protein [XAC(wt)-GFP] in an ATP depletion model. High adenoviral infectivity (70%) in LLC-PK[sub A4.8] cells resulted in linearly increasing XAC(wt)-GFP and phosphorylated (p)XAC(wt)-GFP (inactive) expression. ATP depletion rapidly induced dephosphorylation, and, therefore, activation, of endogenous pcofilin as well as pXAC(wt)-GFP in conjunction with the formation of fluorescent XAC(wt)GFP/actin aggregates and rods. No significant actin cytoskeletal alterations occurred with short-term ATP depletion of LLC-PK[sub A4.8] cells expressing GFP or the constitutively inactive mutant XAC(S3E)-GFP, but cells expressing the constitutively active mutant demonstrated nearly instantaneous actin disruption with aggregate and rod formation. Confocal image three-dimensional volume reconstructions of normal and ATP-depleted LLC-PK[sub A4.8] cells demonstrated that 25 min of ATP depletion induced a rapid increase in XAC(wt)GFP apical and basal signal in addition to XAC-GFP/actin aggregate formation. These data demonstrate XAC(wt)-GFP participates in ischemia-induced actin cytoskeletal alterations and determines the rate and extent of these ATP depletion-induced cellular alterations. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
