التفاصيل البيبلوغرافية
| العنوان: |
Bacterial Community of Koumiss from Mongolia Investigated by Culture and Culture-Independent Methods. |
| المؤلفون: |
Ringø, Einar, Andersen, Renate, Sperstad, Sigmund, Zhou, Zhigang, Ren, Pen, Breines, Eva Marie, Hareide, Ellinor, Yttergård, Gerd Jorun, Opsal, Kirstin, Johansen, Heidi Mathilde, Andreassen, Ann Kristin, Kousha, Armin, Godfroid, Jacques, Holzapfel, Wilhelm |
| المصدر: |
Food Biotechnology; 2014, Vol. 28 Issue 4, p333-353, 21p, 1 Color Photograph, 2 Diagrams, 4 Charts |
| مصطلحات موضوعية: |
BACTERIAL ecology, FERMENTED milk, KUMISS, RIBOSOMAL RNA, HORSE milk, BACTERIAL cultures, GEL electrophoresis |
| مستخلص: |
The bacterial community of fermented horse milk (koumiss) from Mongolia was studied using three methods: cultivation, direct identification by 16S rRNA clone library and denaturing gradient gel electrophoresis (DGGE). Ninety-eight strains were isolated by traditional cultivation and 61 of those were randomly selected for further identification by 16S rRNA gene sequencing. The strains were dominated by lactic acid bacteria (LAB; six different lactobacilli),Acinetobacter, BacillusandPsychrobacter. Construction of the clone library analysis revealed that 16S sequences of 220 clones, genusLactobacilluswas dominant, butStreptococcus thermophilus, Acetobacter pasteurianusand uncultured clones were also detected. Ten unique bands were sequenced from the DGGE and revealed:Lactococcus lactis, Lactococcus lactissubsp.lactis, Clostridium acidurici, Acinetobacter johnsonii, Dickeyasp.,Enterobactersp.,Pseudomonassp.,Raoultellasp., andRuminococcussp..In vitrogrowth inhibition of three human pathogens,Escherichia coli, Enterobacter sakazakiiandStaphylococcus aureusby 14 culturable bacteria displayed that only three of the isolates tested inhibit growth ofE. sakazakiiwhile most of the other bacteria delayed growth of the target bacteria. [ABSTRACT FROM PUBLISHER] |
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| قاعدة البيانات: |
Complementary Index |
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