التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of collection and extraction methodologies of latent fingerprints for military application. |
| المؤلفون: |
Oliveira, T.P., Nogueira, T.L.S., Valentin, E.S.B., Santos, O.C.L., Carvalho, E.F., Silva, D.A. |
| المصدر: |
Forensic Science International: Genetics Supplement Series; Dec2015, Vol. 5, pe474-e475, 1p |
| مصطلحات موضوعية: |
DNA fingerprinting, NUCLEIC acid isolation methods, FORENSIC genetics, CRIME scene searches, ARMIES, POLYMERASE chain reaction |
| مستخلص: |
Latent fingerprint has been commonly used by Brazilian Army forensic experts as an effective tool for personal identification in crime scene investigations. In cases whereas samples are considered unusable for dactyloscopy, genetic analysis appears as a complementary method that prevents waste of expert testimony. The aim of our study was to standardize DNA collection and extraction techniques in two types of surfaces: glass and metal, in order to adapt these methodologies to military reality. Fingerprints were collected using two swabbing solutions: NaCl 0.9% and sodium dodecyl sulfate 2%. DNA was isolated by testing two methods: Lysis solution: SDS/proteinase and QIAmp ® DNA Investigator ® Kit (Qiagen). PCR amplification was performed with AmpFLSTR ® MiniFiler™ Kit (Life Technologies). It was observed that Lysis extraction with NaCl 0.9% swabbing solution gave satisfactory results for samples collected both on glass and metallic supports. [ABSTRACT FROM AUTHOR] |
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