دورية أكاديمية
Transcriptomic profile of key stages of sex differentiation in cassava flowers and discovery of candidate genes related to female flower differentiation.
العنوان: | Transcriptomic profile of key stages of sex differentiation in cassava flowers and discovery of candidate genes related to female flower differentiation. (English) |
---|---|
المؤلفون: | CHEN Hui-Xian, LIANG Zhen-Hua, HUANG Zhen-Ling, WEI Wan-Ling, ZHANG Xiu-Fen, YANG Hai-Xia, LI Heng-Rui, HE Wen, LI Tian-Yuan, LAN Xiu, RUAN Li-Xia, CAI Zhao-Qin, NONG Jun-Xin |
المصدر: | Acta Agronomica Sinica; 2023, Vol. 49 Issue 12, p3250-3260, 11p |
مستخلص: | To solve the breeding problem of the serious shortage of female cassava flowers, cassava variety 'Xinxianxuan 048' was used as the experimental material. Transcriptional sequencing technology was used to analyze the biological information of differentially expressed genes in female and male flowers during the critical period of cassava flower sex differentiation, explore the functions of differentially expressed genes and possible regulatory pathways involved, excavate candidate genes related to female differentiation, and verify the sequencing results by qRT-PCR method. The results showed that: There were 545 differentially expressed genes between male and female cassava flowers at the critical stage of gender differentiation. Among them, 48.63% of the differential genes were enriched in GO pathway of floral organ morphogenesis and development, and the genes enriched in plant phenylpropanol biosynthesis and plant hormone signal transduction were the most. AGL11, YABBY4, CRC, SUP, and other flower sex differentiation genes were significantly up-regulated in female flowers. Four genes of the cytokinin signaling pathway, including HK4, HPt4, ARR8, and ARR12, were significantly up-regulated in female flowers, while IAA14, GH3, SAUR22, and SAURR20, the early auxin response genes, were significantly down-regulated in female flowers. Therefore, the sexual differentiation of cassava flowers mainly involved the biological pathways of pollen, gametophyte, floral organ morphogenesis and development, and two metabolic pathways of plant phenylpropanoid biosynthesis and plant hormone signal transduction. Cytokinin and auxin might be the main hormones in cassava female flower differentiation. AGL11, YABBY4, CRC, SUP, HK4, HPt4, ARR8, and ARR12 may be positive regulators of cassava female flower differentiation. [ABSTRACT FROM AUTHOR] |
Copyright of Acta Agronomica Sinica is the property of Crop Science Society of China and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
قاعدة البيانات: | Supplemental Index |
كن أول من يترك تعليقا!