التفاصيل البيبلوغرافية
| العنوان: |
Rapid differentiation of infectious salmon anemia virus avirulent (HPR0) from virulent (HPRΔ) variants using multiplex RT-qPCR. |
| المؤلفون: |
Rounsville Jr, Thomas F., Polinski, Mark P., Marini, Alyssa G., Turner, Sarah M., Vendramin, Niccolò, Cuenca, Argelia, Pietrak, Michael R., Peterson, Brian C., Bouchard, Deborah A. |
| المصدر: |
Journal of Veterinary Diagnostic Investigation; May2024, Vol. 36 Issue 3, p329-337, 9p |
| مصطلحات موضوعية: |
FISH mortality, ANEMIA, DISEASE management, INTERNAL auditing, SALMON, SALMON farming, PHENOTYPES, ATLANTIC salmon |
| مصطلحات جغرافية: |
NORTH America |
| مستخلص: |
Infectious salmon anemia virus (ISAV; Isavirus salaris) causes an economically important disease of Atlantic salmon (Salmo salar L.). ISA outbreaks have resulted in significant losses of farmed salmon globally, often with a sudden onset. However, 2 phenotypically distinct variants of ISAV exist, each with divergent disease outcomes, associated regulations, and control measures. ISAV-HPRΔ, also known as ISAV-HPR deleted, is responsible for ISA outbreaks; ISAV-HPR0, is avirulent and is not known to cause fish mortality. Current detection methodology requires genetic sequencing of ISAV-positive samples to differentiate phenotypes, which may slow responses to disease management. To increase the speed of phenotypic determinations of ISAV, we developed a new, rapid multiplex RT-qPCR method capable of 1) detecting if a sample contains any form of ISAV, 2) discriminating whether positive samples contain HPRΔ or HPR0, and 3) validating RNA extractions with an internal control, all in a single reaction. Following assay development and optimization, we validated this new multiplex on 31 ISAV strains collected from North America and Europe (28 ISAV-HPRΔ, 3 ISAV-HPR0). Finally, we completed an inter-laboratory comparison of this multiplex qPCR with commercial ISAV testing and found that both methods provided equivalent results for ISAV detection. [ABSTRACT FROM AUTHOR] |
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