التفاصيل البيبلوغرافية
| العنوان: |
Impact of Storage Conditions on Histological and Immunohistochemical Analysis of Rat Skin Samples. |
| المؤلفون: |
Paes, Ms. Stella, Ferreira, Ms. Maria Luiza, Agatti, Ms. Larissa, Netto, Ms. Carolina, Sperandio, Dr. Marcelo, de Araújo, Dr. Vera Cavalcanti, Moraes, Dr. Paulo, de Montalli, Dr. Vera Cavalcanti |
| المصدر: |
Oral Surgery, Oral Medicine, Oral Pathology & Oral Radiology; Aug2024, Vol. 138 Issue 2, pe40-e40, 1p |
| مستخلص: |
The proper preservation of biopsy specimens is crucial for maintaining cellular integrity during histological processing, significantly influencing the quality of immunohistochemical (IHC) and histological analyses. Despite recommendations for 10% buffered formalin as the ideal fixative, pathology labs often receive materials in various solutions. This study assesses the effects of different solutions and temperatures on histological and immunostaining outcomes in IHC reactions, a pivotal diagnostic tool for detecting specific proteins in tissue samples. Skin samples from euthanized Wistar rats were collected and fixed in different solutions (buffered and unbuffered formaldehyde, alcohol, and saline), stored for 24 hours, 7 days, and 30 days. Staining involved Hematoxylin and Eosin, along with Monoclonal Mouse Anti-Human Muscle Actin antibody (Code HHF-35, Dako Denmark). Qualitative and quantitative assessments were conducted. Histological analysis revealed that samples fixed in formalin at room temperature exhibited higher immunostaining and more homogeneous tissue structures than those stored in the refrigerator. Fragments stored in alcohol and saline exhibited reduced immunostaining and tissue autolysis, respectively. Rat skin samples fixed with 10% formalin at room temperature for up to 7 days yielded superior histological and immunohistochemical results, considering cellular expression and integrity. Refrigerated storage increased the incidence of tissue artifacts. This study underscores the importance of optimal storage conditions for preserving tissue quality in histopathological investigations. Valuable insights are provided for pathology laboratories handling diverse fixative solutions, emphasizing the need for adherence to recommended protocols. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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