كتاب
Catalytic Beacons for the Detection of DNA and Telomerase Activity.
| العنوان: | Catalytic Beacons for the Detection of DNA and Telomerase Activity. |
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| المؤلفون: | Morrison, Dennis, Milanovich, Fred, Ivnitski, Dmitri, Austin, Thomas R., Xiao, Y., Pavlov, V., Niazov, T., Dishon, A., Kotler, M., Willner, I. |
| المصدر: | Defense Against Bioterror; 2005, p199-205, 7p |
| مستخلص: | Different new methods for the amplified and specific electronic or photonic detection of DNA will be addressed. Specific methods that will be described include: The amplified electrochemical detection of DNA by means of replication of the analyzed DNA on electrode supports, the incorporation of ferrocene redox-active groups into the replica, and the secondary activation of bioelectrocatalytic cascades.The use of rotating magnetic particles as sensing matrices for DNA using electrogenerated chemiluminescence as an optical readout signal.The magneto-mechanical detection of DNA on cantilever sensors exposed to an external magnetic field. In these systems, magnetic particles are attached to the target-replicated DNA associated with the lever, and the lever is mechanically deflected when subjected to an external magnet.The use of semiconductor quantum dots (QDs) for the photonic detection of DNA will be described by using nucleic acid-functionalized QDs that hybridize with the analyte DNA (CdSe-ZnS or CdTe quantum dots). The replication of the analyzed DNA while incorporating an appropriate dye allows, in the next step, to confirm the hybridization of the analyte DNA by fluorescence resonance energy transfer (FRET). The parallel detection of different DNA/RNA will be described by using QDs of different sizes and compositions.Catalytic nucleic acids that bind hemin and mimic peroxidase activity were employed for the amplified detection of DNA (or proteins). The DNAzyme leads to the generation of chemiluminescence in the presence of luminol/H2O2. Different DNAzyme-based catalytic schemes for the detection of DNA will be described, including the use of catalytic beacons and Au-nanoparticle/DNAzyme hybrid systems. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: | Supplemental Index |
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