دورية
The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase.
العنوان: | The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase. |
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المؤلفون: | Houghton, M, Easton, M A, Stewart, A G, Smith, J C, Doel, S M, Catlin, G H, Lewis, H M, Patel, T P, Emtage, J S, Carey, N H, Porter, A G |
المصدر: | Nucleic Acids Research; July 1980, Vol. 8 Issue: 13 p2885-2894, 10p |
مستخلص: | Using synthetic oligodeoxyribonucleotides to prime the transcription of interferon mRNA and cDNA, we recently determined the mRNA sequence coding for the 47 amino-terminal amino acids of mature human fibroblast interferon (1). From this sequence, we have now synthesised an oligodeoxyribonucleotide that is homologous with the mRNA sequence coding for amino acids 42-45 and used it as a primer to selectively transcribe an interferon cDNA template. The sequence of the newly synthesised DNA predicted the sequence of amino acids 48-109 in the interferon polypeptide. By repeating this process with one more primer, we have determined the complete amino acid sequence of mature human fibroblast interferon, a polypeptide of 166 amino acids. |
قاعدة البيانات: | Supplemental Index |
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