دورية
The Use of HIS6Gene as a Selectable Marker for Yeast Vector
| العنوان: | The Use of HIS6Gene as a Selectable Marker for Yeast Vector |
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| المؤلفون: | IMADEARTIKA |
| المصدر: | HAYATI Journal of Biosciences; March 2009, Vol. 16 Issue: 1 p40-42, 3p |
| مستخلص: | The yeast Saccharomyces cerevisiae HIS6gene has been shown to be functional as a selectable marker for selecting and maintaining a yeast vector in yeast S. cerevisiaehost cells. The yeast HIS6gene encodes an enzyme involved in the yeast histidine biosynthesis. The yeast HIS6gene was cloned into a yeast expression vector. The resultant recombinant plasmid was introduced into yeast host cells defective in endogenous HIS6gene. The functionality of the HIS6gene as a selectable marker was tested by growing transformed cells on selective minimum medium lacking histidine supplementation. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 20864094 19783019 |
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| DOI: | 10.4308/hjb.16.1.40 |