دورية
In VitroSelection of Abaca for Resistance to Fusarium oxysporumf.sp. cubense
| العنوان: | In VitroSelection of Abaca for Resistance to Fusarium oxysporumf.sp. cubense |
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| المؤلفون: | PURWATI, RULLY DYAH, HARRAN, SAID, SUDARSONO |
| المصدر: | HAYATI Journal of Biosciences; June 2007, Vol. 14 Issue: 2 p65-70, 6p |
| مستخلص: | Abaca (Musa textilisNee) is an important industrial crop. However, the cultivation of this crop in Indonesia is hampered by Fusariumwilt (Panama disease) as a result of Fusarium oxysporumf.sp. cubense(Foc) infection. The objectives of this study were to (i) evaluate inhibitory effects of culture filtrates (CF) of three Focisolates (Banyuwangi, Malang, and Bojonegoro isolates) on shoot growth of abaca cv. Tangongon and Sangihe-1, (ii) determine sublethal concentration of FocCF, (iii) isolate variant cells/tissues which are insensitive against FocCF and regenerate plantlets from the variants, and (iv) evaluate responses of the plantlets against Focinfection. The results of the experiment showed that even though CF of all Focisolates inhibited abaca's shoot growth, CF of FocBanyuwangi isolate showed the most inhibitory effect. Sublethal concentration of CF of FocBanyuwangi isolate was 40%. From abaca cv. Tangongon, 326 shoots were regenerated from CF insensitive embryogenic calli while from Sangihe-1 – 176 shoots were regenerated. Following acclimatization and Focinoculation using detached-leaf dual culture test, a total of four immune, two resistant, and two moderately resistant plantlets were identified out of 45 tested variants of Tangongon. On the other hand, only two resistant and one moderately resistant plantlets were identified out of 10 tested variants of Sangihe-1. |
| قاعدة البيانات: | Supplemental Index |
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