دورية
Activation of recA protein: the pitch of the helical complex with single‐stranded DNA.
| العنوان: | Activation of recA protein: the pitch of the helical complex with single‐stranded DNA. |
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| المؤلفون: | Hewat, E.A., Ruigrok, R.W., DiCapua, E. |
| المصدر: | EMBO Journal; September 1991, Vol. 10 Issue: 9 p2695-2698, 4p |
| مستخلص: | The complex of recA protein with single‐stranded DNA in the presence of ATP is the active species in the three enzymatic activities of recA: the initiation of strand exchange, the hydrolysis of ATP and the cleavage of repressors. Here we find by cryo‐electron microscopy of unstained and unfixed samples that the helical structure of the protein coat in this complex differs slightly but significantly from the structure in the complex with double‐stranded DNA. We discuss how the larger pitch of the complex with single strands (100 +/‐ 2 A compared with 95 +/‐ 2 A with double strands) could contribute to its higher enzymatic activity. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 02614189 14602075 |
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| DOI: | 10.1002/j.1460-2075.1991.tb07813.x |