التفاصيل البيبلوغرافية
| العنوان: |
T lymphocyte subpopulations and B lymphocyte cellsin caecum and spleen of chicks infected with Salmonella enteritidis |
| المؤلفون: |
Asheg, Abdulatif, Levkut, Mikuláš, Revajová, Viera, Ševčíková, Zuzana, Kolodzieyski, Lev, Pistl, Juraj |
| المصدر: |
Acta Histochemica; January 2002, Vol. 104 Issue: 4 p435-439, 5p |
| مستخلص: |
The effect of low and high doses of Salmonella enteritidisPT4 (SE) on immunocompetent cells in caecum and spleen of one-day-old chicks was investigated. Subsets of T lymphocytes positive for CD3, CD4, CD8 and B lymphocytes (Bu1b-positive cells) were counted in the caecum after immunohistochemical staining and the relative percentage of these cells in the spleen was analysed using a FACScan cytometer on days 7, 10, 14, 21, and 27 post-inoculation (pi). In the low dose group, the number of CD3+ and CD4+ cells in the caecum had significantly increased at day 10 pi. Both CD8+ and Bu1b+ cells were significantly higher on day 14 pi in this group. In the high-dose group, the number of CD4+ cells had significantly increased at day 7 pi. CD3+, CD8+, and Bu1b+ cells showed prolonged proliferation at days 7 up to 21 pi. Splenic lymphocytes demonstrated significant changes only in the high dose group. The percentage of splenic CD4+ cells was decreased at day 7 pi. A decrease in CD3+ and CD8+ cells was found at day 14 pi in this group. |
| قاعدة البيانات: |
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