دورية
Activation of the α-Internexin Promoter by the Brn-3a Transcription Factor Is Dependent on the N-terminal Region of the Protein (∗)
| العنوان: | Activation of the α-Internexin Promoter by the Brn-3a Transcription Factor Is Dependent on the N-terminal Region of the Protein (∗) |
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| المؤلفون: | Budhram-Mahadeo, Vishwanie, Morris, Peter J., Lakin, Nic D., Theil, Thomas, Ching, Gee Y., Lillycrop, Karen A., Möröy, Tarik, Liem, Ronald K.H., Latchman, David S. |
| المصدر: | Journal of Biological Chemistry; February 1995, Vol. 270 Issue: 6 p2853-2858, 6p |
| مستخلص: | The Brn-3a, Brn-3b, and Brn-3c proteins are closely related POU (Pit-Oct-Unc) family transcription factors which are expressed predominantly in neuronal cells. We have identified the α-internexin gene as the first reported, neuronally expressed, target gene whose promoter activity is modulated by these factors. Both the Brn-3a and Brn-3c factors can activate the α-internexin promoter while Brn-3b represses it and can prevent activation by Brn-3a. Using chimeric constructs containing different regions of Brn-3a or Brn-3b, we show that activation of the α-internexin promoter requires the N-terminal region of Brn-3a. In contrast the activation by Brn-3a but not Brn-3b of an artificial promoter containing a synthetic Brn-3 binding site can be shown using the same constructs to be dependent on the POU domain of Brn-3a. Moreover, the isolated POU domain of Brn-3a can activate this artificial promoter but not the α-internexin promoter. Hence Brn-3a contains two distinct transactivation domains, at the N terminus and within the POU domain, whose effect is dependent upon the target promoter. The relationship of gene transactivation by Brn-3a to its ability to transform primary cells which is also dependent on the N-terminal region of the protein is discussed. |
| قاعدة البيانات: | Supplemental Index |
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