دورية
Sar1bmutant mice recapitulate gastrointestinal abnormalities associated with chylomicron retention disease
| العنوان: | Sar1bmutant mice recapitulate gastrointestinal abnormalities associated with chylomicron retention disease |
|---|---|
| المؤلفون: | Auclair, Nickolas, Sané, Alain T., Ahmarani, Lena, Patey, Nathalie, Beaulieu, Jean-François, Peretti, Noel, spahis, Schohraya, Levy, Emile |
| المصدر: | Journal of Lipid Research; 20210101, Issue: Preprints |
| مستخلص: | Chylomicron retention disease (CRD) is an autosomal recessive disorder associated with biallelic Sar1bmutations leading to defects in intracellular chylomicron (CM) trafficking and secretion. To date, a direct cause-effect relationship between CRD and Sar1bmutation has not been established, but genetically modified animal models provide an opportunity to elucidate unrecognized aspects of these mutations. To examine the physiological role and molecular mechanisms of Sar1b function, we generated mice expressing either a targeted deletion or mutation of human Sar1busing the CRISPR/Cas9 system. We found that deletion or mutation of Sar1bin mice resulted in late-gestation lethality of homozygous embryos. Moreover, compared to WT mice, heterozygotes carrying a single disrupted Sar1ballele displayed lower plasma levels of triglycerides, total cholesterol, and HDL-cholesterol, along with reduced CM secretion following gastric lipid gavage. Similarly, decreased expression of apolipoprotein B and microsomal triglyceride transfer protein was observed in correlation with the accumulation of mucosal lipids. Inefficient fat absorption in heterozygotes was confirmed via an increase in fecal lipid excretion. Furthermore, genetically modified Sar1baffected intestinal lipid homeostasis as demonstrated by enhanced fatty acid β-oxidation and diminished lipogenesis through the modulation of transcription factors. This is the first reported mammalian animal model with human Sar1bgenetic defects, which reproduces some of the characteristic CRD features and provides a direct cause-effect demonstration. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00222275 15397262 |
|---|---|
| DOI: | 10.1016/j.jlr.2021.100085 |