دورية
Intranasal Vaccination with Chitosan-DNA Nanoparticles Expressing Pneumococcal Surface Antigen A Protects Mice against Nasopharyngeal Colonization by Streptococcus pneumoniae
| العنوان: | Intranasal Vaccination with Chitosan-DNA Nanoparticles Expressing Pneumococcal Surface Antigen A Protects Mice against Nasopharyngeal Colonization by Streptococcus pneumoniae |
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| المؤلفون: | Xu, Jianghong, Dai, Wenjia, Wang, Zhengmin, Chen, Bing, Li, Zhongming, Fan, Xiaoyong |
| المصدر: | Clinical and Vaccine Immunology (formerly CDLI); November 2010, Vol. 18 Issue: 1 p75-81, 7p |
| مستخلص: | ABSTRACTStreptococcus pneumoniaeis a respiratory pathogen, and mucosal immune response plays a significant role in the defense against pneumococcal infections. Thus, intranasal vaccination may be an alternative approach to current immunization strategies, and effective delivery systems to mucosal organism are necessary. In this study, BALB/c mice were immunized intranasally with chitosan-DNA nanoparticles expressing pneumococcal surface antigen A (PsaA). Compared to levels in mice immunized with naked DNA or chitosan-pVAX1, anti-PsaA IgG antibody in serum and anti-IgA antibody in mucosal lavages were elevated significantly in mice immunized with chitosan-psaA. The balanced IgG1/IgG2a antibody ratio in serum, enhanced gamma interferon (IFN-?) and IL-17A levels in spleen lymphocytes, and mucosal washes of mice immunized with chitosan-psaAsuggested that cellular immune responses were induced. Furthermore, significantly fewer pneumococci were recovered from the nasopharynx of mice immunized with chitosan-psaAthan for the control group following intranasal challenge with ATCC 6303 (serotype 3). These results demonstrated that mucosal immunization with chitosan-psaAmay successfully generate mucosal and systemic immune responses and prevent pneumococcal nasopharyngeal colonization. Hence, a chitosan-DNA nanoparticle vaccine expressing pneumococcal major immunodominant antigens after intranasal administration could be developed to prevent pneumococcal infections. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 15566811 1556679X |
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| DOI: | 10.1128/CVI.00263-10 |