التفاصيل البيبلوغرافية
| العنوان: |
Expanding Knowledge of Methylotrophic Capacity: Structure and Properties of the Rough-Type Lipopolysaccharide from Methylobacterium extorquensand Its Role on Membrane Resistance to Methanol |
| المؤلفون: |
Di Lorenzo, Flaviana, Nicolardi, Simone, Marchetti, Roberta, Vanacore, Adele, Gallucci, Noemi, Duda, Katarzyna, Nieto Fabregat, Ferran, Nguyen, Ha Ngoc Anh, Gully, Djamel, Saenz, James, Giraud, Eric, Paduano, Luigi, Molinaro, Antonio, D’Errico, Gerardino, Silipo, Alba |
| المصدر: |
JACS Au; March 2023, Vol. 3 Issue: 3 p929-942, 14p |
| مستخلص: |
The ability of Methylobacterium extorquensto grow on methanol as the sole carbon and energy source has been the object of intense research activity. Unquestionably, the bacterial cell envelope serves as a defensive barrier against such an environmental stressor, with a decisive role played by the membrane lipidome, which is crucial for stress resistance. However, the chemistry and the function of the main constituent of the M. extorquensouter membrane, the lipopolysaccharide (LPS), is still undefined. Here, we show that M. extorquensproduces a rough-type LPS with an uncommon, non-phosphorylated, and extensively O-methylated core oligosaccharide, densely substituted with negatively charged residues in the inner region, including novel monosaccharide derivatives such as O-methylated Kdo/Ko units. Lipid A is composed of a non-phosphorylated trisaccharide backbone with a distinctive, low acylation pattern; indeed, the sugar skeleton was decorated with three acyl moieties and a secondary very long chain fatty acid, in turn substituted by a 3-O-acetyl-butyrate residue. Spectroscopic, conformational, and biophysical analyses on M. extorquensLPS highlighted how structural and tridimensional features impact the molecular organization of the outer membrane. Furthermore, these chemical features also impacted and improved membrane resistance in the presence of methanol, thus regulating membrane ordering and dynamics. |
| قاعدة البيانات: |
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