دورية
Contribution of YPRO15COverexpression to the Resistance of Saccharomyces cerevisiaeBY4742 Strain to Furfural Inhibitor
| العنوان: | Contribution of YPRO15COverexpression to the Resistance of Saccharomyces cerevisiaeBY4742 Strain to Furfural Inhibitor |
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| المؤلفون: | Abrha, Getachew Tafere, Li, Qian, Kuang, Xiaolin, Xiao, Difan, Ayepa, Ellen, Wu, Jinjian, Chen, Huan, Zhang, Zhengyue, Liu, Yina, Yu, Xiumei, Xiang, Quanju, Ma, Menggen |
| المصدر: | Polish Journal of Microbiology; June 2023, Vol. 72 Issue: 2 p177-186, 10p |
| مستخلص: | Lignocellulosic biomass is still considered a feasible source of bioethanol production. Saccharomyces cerevisiaecan adapt to detoxify lignocellulose-derived inhibitors, including furfural. Tolerance of strain performance has been measured by the extent of the lag phase for cell proliferation following the furfural inhibitor challenge. The purpose of this work was to obtain a tolerant yeast strain against furfural through overexpression of YPR015Cusing the in vivohomologous recombination method. The physiological observation of the overexpressing yeast strain showed that it was more resistant to furfural than its parental strain. Fluorescence microscopy revealed improved enzyme reductase activity and accumulation of oxygen reactive species due to the harmful effects of furfural inhibitor in contrast to its parental strain. Comparative transcriptomic analysis revealed 79 genes potentially involved in amino acid biosynthesis, oxidative stress, cell wall response, heat shock protein, and mitochondrial-associated protein for the YPR015Coverexpressing strain associated with stress responses to furfural at the late stage of lag phase growth. Both up- and down-regulated genes involved in diversified functional categories were accountable for tolerance in yeast to survive and adapt to the furfural stress in a time course study during the lag phase growth. This study enlarges our perceptions comprehensively about the physiological and molecular mechanisms implicated in the YPR015Coverexpressing strain’s tolerance under furfural stress. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 17331331 25444646 |
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| DOI: | 10.33073/pjm-2023-019 |