دورية
Fosfomycin activity in vitroagainst Escherichia colistrains isolated from urine specimens
العنوان: | Fosfomycin activity in vitroagainst Escherichia colistrains isolated from urine specimens |
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المؤلفون: | Zdzieblo, Magdalena, Biernasiuk, Anna, Helon, Pawel, Malm, Anna |
المصدر: | Current Issues in Pharmacy and Medical Sciences; December 2023, Vol. 36 Issue: 4 p217-220, 4p |
مستخلص: | Urinary tract infections are caused mostly by uropathogenic Escherichia colistrains. The aim of this paper was to assess the activity of fosfomycin in vitroagainst 74 E. colistrains isolated from urine samples of outpatients with acute cystitis. Minimum inhibitory concentration (MIC) was determined by the recommended method of serial drug dilution in Mueller-Hinton agar supplemented with glucose-6-phosphate (25 mg/L). The estimated MIC values were in the range of 1-32 mg/L. The prevalence of fosfomycin-sensitive strains was 45.9%, while that of fosfomycin-resistant strains – 54.1%. The time-kill assay was performed for a chosen clinical strain (MIC = 1 mg/L) in Mueller-Hinton broth also supplemented with glucose-6-phosphate in the presence of various concentrations of fosfomycin (0.5-64 mg/L). The bacterial population density was expressed as log CFU (colony forming units)/mL. The decrease of the bacterial population viability after 6h incubation was found with ∆log CFU/mL = 1.05-5.02, depending on fosfomycin concentration. However, bactericidal effect of this antibiotic (∆log CFU/mL >3) was observed only at the highest concentrations (32-64 mg/L). Moreover, after prolonged incubation (24 h), the re-growth of bacterial population revealed as the increase of its viability (∆log CFU/mL = 1.03-3.03) was observed at fosfomycin concentration in the range 0.5-32 mg/L, but not at 64 mg/L. This phenomenon may be due the presence of subpopulations of spontaneous mutants sensitive only to higher concentrations of this antibiotic comparing to MIC. The presented data confirm a need to monitor the sensitivity of uropathogenic E. colistrains to fosfomycin. |
قاعدة البيانات: | Supplemental Index |
تدمد: | 23006676 2084980X |
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DOI: | 10.2478/cipms-2023-0037 |