The novel genus, ‘CandidatusPhosphoribacter’, previously identified as Tetrasphaera, is the dominant polyphosphate accumulating lineage in EBPR wastewater treatment plants worldwide

التفاصيل البيبلوغرافية
العنوان: The novel genus, ‘CandidatusPhosphoribacter’, previously identified as Tetrasphaera, is the dominant polyphosphate accumulating lineage in EBPR wastewater treatment plants worldwide
المؤلفون: Singleton, C M, Petriglieri, F, Wasmund, K, Nierychlo, M, Kondrotaite, Z, Petersen, J F, Peces, M, Dueholm, M S, Wagner, M, Nielsen, P H
المصدر: The ISME Journal; June 2022, Vol. 16 Issue: 6 p1605-1616, 12p
مستخلص: The bacterial genus Tetrasphaeraencompasses abundant polyphosphate accumulating organisms (PAOs) that are responsible for enhanced biological phosphorus removal (EBPR) in wastewater treatment plants. Recent analyses of genomes from pure cultures revealed that 16S rRNA genes cannot resolve the lineage, and that Tetrasphaeraspp. are from several different genera within the Dermatophilaceae. Here, we examine 14 recently recovered high-quality metagenome-assembled genomes from wastewater treatment plants containing full-length 16S rRNA genes identified as Tetrasphaera, 11 of which belong to the uncultured Tetrasphaeraclade 3. We find that this clade represents two distinct genera, named here Ca. Phosphoribacter and Ca. Lutibacillus, and reveal that the widely used model organism Tetrasphaera elongatais less relevant for physiological predictions of this uncultured group. Ca. Phosphoribacter incorporates species diversity unresolved at the 16S rRNA gene level, with the two most abundant and often co-occurring species encoding identical V1-V3 16S rRNA gene amplicon sequence variants but different metabolic capabilities, and possibly, niches. Both Ca. P. hodrii and Ca. P. baldrii were visualised using fluorescence in situ hybridisation (FISH), and PAO capabilities were confirmed with FISH-Raman microspectroscopy and phosphate cycling experiments. Ca. Phosphoribacter represents the most abundant former Tetrasphaeralineage and PAO in EPBR systems in Denmark and globally.
قاعدة البيانات: Supplemental Index
الوصف
تدمد:17517362
17517370
DOI:10.1038/s41396-022-01212-z