دورية
CandidatusRickettsia senegalensis in Cat Fleas (Siphonaptera: Pulicidae) Collected from Dogs and Cats in Cauca, Colombia
| العنوان: | CandidatusRickettsia senegalensis in Cat Fleas (Siphonaptera: Pulicidae) Collected from Dogs and Cats in Cauca, Colombia |
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| المؤلفون: | Betancourt-Ruiz, Paola, Martínez-Díaz, Heidy-C, Gil-Mora, Juliana, Ospina, Catalina, Olaya-M, Luz-Adriana, Benavides, Efraín, Bolaños, Eliana, Cuervo, Claudia, Blanton, Lucas, Hidalgo, Marylin |
| المصدر: | Journal of Medical Entomology; March 2020, Vol. 57 Issue: 2 p382-387, 6p |
| مستخلص: | Rickettsia typhiand Rickettsia felis(Rickettsiales: Rickettsiaceae) are flea-transmitted pathogens. They are important causes of acute febrile illness throughout the world. We, therefore, sought to identify the rickettsial species present in the fleas of dogs and cats in the department of Cauca, Colombia. In this study, we collected 1,242 fleas from 132 dogs and 43 fleas from 11 cats. All fleas were morphologically identified as Ctenocephalides felis(Bouché) adults and organized in pools for DNA extraction (234 pools from dogs and 11 from cats). The gltAgene from rickettsiae was targeted for screening amplification using conventional PCR. In total, 144 of the 245 pools (58.7%) were positive. The positive samples were then processed for the amplification of the 17kDa antigen gene (144/144; 100% positive) and sca5gene (140/144; 97.2% positive). In addition, restriction enzyme length polymorphism analysis using NlaIV on the amplified product of the sca5gene demonstrated several organisms: 21/140 (15%) were R. felis, 118/140 (84.3%) were Rickettsia asemboensis,and 1/140 (0.7%) were CandidatusRickettsia senegalensis. Subsequent sequencing confirmed CandidatusRickettsia senegalensis in C. feliscollected from dogs the first reported from Colombia. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00222585 19382928 |
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| DOI: | 10.1093/jme/tjz177 |