دورية
An Anti-okadaic Acid-Anti-idiotypic Antibody Bearing An Internal Image of Okadaic Acid Inhibits Protein Phosphatase PP1 and PP2A Catalytic Activity
| العنوان: | An Anti-okadaic Acid-Anti-idiotypic Antibody Bearing An Internal Image of Okadaic Acid Inhibits Protein Phosphatase PP1 and PP2A Catalytic Activity |
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| المؤلفون: | Shestowsky, W.S., Holmes, C.F.B., Hu, T.M., Marr, J., Wright, J.L.C., Chin, J., Sikorska, H.M. |
| المصدر: | Biochemical and Biophysical Research Communications; April 1993, Vol. 192 Issue: 1 p302-310, 9p |
| مستخلص: | Okadaic acid (OA), produced by marine phytoplankton, is the parent compound of a family of marine toxins responsible for diarrhetic shellfish poisoning (DSP). A monoclonal antibody to OA (6/50) (Ab1) has been raised and in turn used for immunization of syngeneic animals. Mice inoculated with the 6/50 idiotype produced both anti-idiotypic antibodies (Ab2) and OA binding antibodies (Ab3). The selected anti-idiotypic antibody 1/59 bound to the immunizing 6/50 idiotype but not to F(ab′)2fragments of pooled normal mouse Ig. It inhibited the binding of OA to solid-phase attached F(ab′)2of 6/50 IgG as well as the binding of 6/50 IgG to a solid-phase bound OA. Like OA, 1/59 anti-idiotypic antibody inhibited protein phosphatase 1 and 2A catalytic subunits in a 32P-phosphorylase aphosphatase radioassay. Thus, 1/59 IgG is a novel internal image anti-idiotypic antibody (Ab2β) and can serve as a surrogate of OA in biological assays. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 0006291X 10902104 |
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| DOI: | 10.1006/bbrc.1993.1414 |