An Electrochemical Biosensor with Cholesterol Oxidase/ Sol‐Gel Film on a Nanoplatinum/Carbon Nanotube Electrode

التفاصيل البيبلوغرافية
العنوان: An Electrochemical Biosensor with Cholesterol Oxidase/ Sol‐Gel Film on a Nanoplatinum/Carbon Nanotube Electrode
المؤلفون: Qiaocui, Shi, Tuzhi, Peng, Yunu, Zhu, Yang, Catherine F.
المصدر: Electroanalysis; May 2005, Vol. 17 Issue: 10 p857-861, 5p
مستخلص: The carbon nanotubes decorated nanoplatinum (CNT‐Pt) were prepared using a chemical reduction method and a novel base electrode was constructed by intercalating CNT‐Pt on the surface of a waxed graphite electrode. The results showed that the nano‐particles of platinum at a waxed graphite electrode exhibits high catalytic activity for the reduction of hydrogen peroxide. The cholesterol oxidase (ChOx), chosen as a model enzyme, was immobilized with sol‐gel on the CNT‐Pt base electrode to construct a biosensor. The current response of the biosensor for cholesterol was very rapid (<20 s). The linear range for cholesterol measurement was 4.0×10−6mol/L −1.0×10−4mol/L with a detection limit of 1.4×10−6mol/L. The experiments also showed that the ChOx/sol‐gel/CNT‐Pt biosensor was sensitive and stable in detecting cholesterol in serum samples.
قاعدة البيانات: Supplemental Index
الوصف
تدمد:10400397
15214109
DOI:10.1002/elan.200403162