دورية
Determination of cholesterol at the low picomole level by nano-electrospray ionization tandem mass spectrometry.
| العنوان: | Determination of cholesterol at the low picomole level by nano-electrospray ionization tandem mass spectrometry. |
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| المؤلفون: | Sandhoff, R, Brügger, B, Jeckel, D, Lehmann, W D, Wieland, F T |
| المصدر: | Journal of Lipid Research; January 1999, Vol. 40 Issue: 1 p126-32, 7p |
| مستخلص: | A mass spectrometric method for the quantification of free cholesterol in cells and subcellular membranes is presented. The method is based on a simple one-step chemical derivatization of cholesterol to cholesterol-3-sulfate by a sulfur trioxide-pyridine complex. Quantification is performed by nano-electrospray ionization tandem mass spectrometry (nanoESI-MS/MS) using a stable isotope labeled internal standard. The determination of free cholesterol is demonstrated in about 250 cells of a Chinese hamster ovary (CHO) cell line. With this method a molar ratio of free cholesterol to total phospholipids of 0.34 mol/mol in CHO cells was determined. In a subcellular membrane fraction enriched in Golgi membranes, a molar ratio of free cholesterol to total phospholipids of 0.57 mol/mol was determined. The method should be of value for quantification of other sterols as demonstrated for ergosterol and stigmasterol. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00222275 15397262 |
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