دورية
Changes in the expression of MCP-1 receptors on monocytic THP-1 cells following differentiation to macrophages with phorbol myristate acetate
| العنوان: | Changes in the expression of MCP-1 receptors on monocytic THP-1 cells following differentiation to macrophages with phorbol myristate acetate |
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| المؤلفون: | Denholm, Elizabeth M., Stankus, Gerald P. |
| المصدر: | Cytokine; July, 1995, Vol. 7 Issue: 5 p436-440, 5p |
| مستخلص: | Human monocytic THP-1 cells were differentiated to macrophages by incubation with 1.0 μM phorbol myristate acetate (PMA) for 1 to 18 h; cells were then assayed for the ability to migrate to MCP-1. In comparison to undifferentiated monocytes, the chemotactic response of PMA-differentiated cells to MCP-1 decreased with treatment time. This loss of the chemotactic response to MCP-1 correlated with increased in cellular enzymes characteristic of differentiated macrophages. Receptors binding assays demonstrated a parallel decrease in specific binding of MCP-1 with increased incubation with PMA. Undifferentiated monocytes had 1175 ± 387 receptors per cell with a Kd of 1.53 ± 0.35 nM. Cells differentiated to macrophages with PMA rapidly lost the ability to bind MCP-1, with a significant decrease apparent following 3 h incubation with PMA. The reduction in specific binding of MCP-1 by MΦ-THP-1 cells was due to a decrease in both receptor number and affinity; receptor number was reduced to 481 ± 106 receptors/cells with a Kd of 3.16 ± 0.7 nM on cells treated for 3 h with PMA. The demonstrated changes in receptor affinity and expression with differentiation may be a mechanism of controlling macrophage responsiveness to chemokines in inflammatory foci. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 10434666 10960023 |
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| DOI: | 10.1006/cyto.1995.0059 |