Protein Phosphorylation Dynamics: Unexplored Because of Current Methodological Limitations
| العنوان: | Protein Phosphorylation Dynamics: Unexplored Because of Current Methodological Limitations |
|---|---|
| المؤلفون: | Robichon, Alain |
| المساهمون: | Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), French National Research Agency (ANR)ANR12BSV6000601French National Research Agency (ANR)ANR-11-LABX-002801 |
| المصدر: | BioEssays BioEssays, Wiley-VCH Verlag, 2020, 42 (4), pp.1900149. ⟨10.1002/bies.201900149⟩ |
| بيانات النشر: | HAL CCSD, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | MS analysis, kinases, phosphoproteome, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, phosphatases, transient complexes |
| الوصف: | The study of intrinsic phosphorylation dynamics and kinetics in the context of complex protein architecture in vivo has been challenging: Method limitations have prevented significant advances in the understanding of the highly variable turnover of phosphate groups, synergy, and cooperativity between P-sites. However, over the last decade, powerful analytical technologies have been developed to determine the full catalog of the phosphoproteome for many species. The curated databases of phospho sites found by mass spectrometry analysis and the computationally predicted sites based on the linear sequence of kinase motifs are valuable tools. They allow investigation of the complexity of phosphorylation in vivo, albeit with strong discrepancies between different methods. A series of hypothetical scenarios on combinatorial processive phosphorylation is proposed that are likely unverifiable with current methodologies. These proposed a priori postulates could be considered as possible extensions of the known schemes of the activation/inhibition signaling process in vivo. |
| اللغة: | English |
| تدمد: | 0265-9247 1521-1878 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::ce7103845ee1f59fbc4d4a2a5bc30c56 https://hal.inrae.fr/hal-02959977 |
| رقم الأكسشن: | edsair.dedup.wf.001..ce7103845ee1f59fbc4d4a2a5bc30c56 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 02659247 15211878 |
|---|