R11 peptides can promote the molecular imaging of spherical nucleic acids for bladder cancer margin identification
| العنوان: | R11 peptides can promote the molecular imaging of spherical nucleic acids for bladder cancer margin identification |
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| المؤلفون: | Mengzhao Zhang, Jinhai Fan, Lei Wang, Lu Wang, Yuhang Chen, Qiuya Shao, Minghai Ma, Daxiang Cui, Weichao Dan, Jiatao Hao, Pu Zhang, Lu Zhang, Xi Liu, Xiao Liang, Haibao Zhang, Rundong Song, Tao Yang, Nan Zhang, Lijiang Gu, Minxuan Jing |
| المصدر: | Nano Research. 15:2278-2287 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Fluorescence-lifetime imaging microscopy, Nuclease, Bladder cancer, biology, Chemistry, RNA, Condensed Matter Physics, medicine.disease, Atomic and Molecular Physics, and Optics, Molecular beacon, Survivin, biology.protein, Cancer research, Nucleic acid, medicine, General Materials Science, Electrical and Electronic Engineering, Molecular imaging |
| الوصف: | One of the critical problems in bladder cancer (BC) management is the local recurrence of disease. However, achieving the accurate delineation of tumor margins intraoperatively remains extremely difficult due to the lack of effective tumor margin recognition technology. Herein, survivin molecular beacon (MB) and R11 peptide-linked spherical nucleic acids (SNAs) were synthesized as nanoprobes (AuNP-MB@R11) for sensitive detection of BC margins. Physicochemical properties proved that R11 peptides and survivin MB were successfully loaded onto the surface of SNAs. AuNP-MB@R11 had good stability against nuclease activity and high sensitivity and specificity to detect survivin single strand DNA (ssDNA) in vitro. According to cytology, R11 peptides could increase the BC targeting ability and membrane penetrability of SNAs. Notably, R11 peptides significantly promoted the disintegration of lysosomes and the release of SNAs to enhance fluorescence imaging quality. Further RNA sequencing proved that some genes and pathways related to endocytosis and lysosomes were significantly regulated, such as AGPAT5, GPD1L, and GRB2. In orthotopic BC models and a clinical sample from a patient with BC, AuNP-MB@R11 showed a more legible cancerous fluorescence margin and offered remarkably improved detection compared to those achieved by SNAs. R11 peptide-linked SNAs present promising potential to identify BC margin, which may help to improve the R0 resection rate in surgery and improve patients’ quality of life. |
| تدمد: | 1998-0000 1998-0124 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::025d8caf1d168f9328cf84cb5128a8f5 https://doi.org/10.1007/s12274-021-3807-z |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........025d8caf1d168f9328cf84cb5128a8f5 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 19980000 19980124 |
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